| Date | R | Home v Away | - | |
|---|---|---|---|---|
| 12/28 15:00 | - | Harlequins Women v Leicester Tigers Women | W | 42-17 |
| 12/14 15:05 | - | Loughborough Lightning Women v Harlequins Women | W | 22-40 |
| 11/24 15:00 | - | Saracens Women v Harlequins Women | W | 14-15 |
| 03/08 19:45 | - | Gloucester Hartpury Women v Harlequins Women | L | 57-24 |
| 02/10 12:30 | - | Saracens Women v Harlequins Women | L | 29-24 |
| 02/04 15:00 | - | Harlequins Women v Sale Sharks Women | W | 53-12 |
| 12/30 14:30 | - | Harlequins Women v Gloucester Hartpury Women | L | 19-31 |
| 12/09 15:00 | - | Harlequins Women v Exeter Chiefs Women | L | 27-52 |
| 05/21 13:30 | - | Gloucester Hartpury Women v Harlequins Women | L | 67-14 |
| 03/11 12:30 | - | Bristol Bears Women v Harlequins Women | L | 31-26 |
| 03/04 14:30 | - | Harlequins Women v Exeter Chiefs Women | L | 25-54 |
| 01/22 14:30 | - | Harlequins Women v Gloucester Hartpury Women | L | 22-33 |
| 12/27 13:30 | - | Harlequins Women v Bristol Bears Women | W | 48-14 |
| 05/22 13:00 | - | Saracens Women v Harlequins Women | L | 30-10 |
| 12/27 14:15 | - | Harlequins Women v Wasps Ladies | W | 29-5 |
| 05/30 15:00 | - | Saracens Women v Harlequins Women | W | 17-25 |
| 04/27 16:45 | - | Saracens Women v Harlequins Ladies | L | 30-17 |
| 04/29 13:57 | - | Saracens Women v Harlequins Ladies | L | 24-20 |